ELECTROPHORESIS



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Introduction:

Electrophoresis is considered to be one of the worlds leading technologies for analytical and preparative methods and for innovative applications in all areas of electrophoresis. In life sciences, electrophoresis is also one of the most ingenious and important methods with ubiquitous applications within both research and routine.

Continuing to serve as an indispensable vehicle for the dissemination of efficacious, Electrophoresis advances by covering all operative approaches from gels through capillaries to chips.

Instrumentation:

The apparatus of an elecrode consistes of a high-voltage supply, elecrodes, buffer and a support for the buffer such a filter paper, cellulose acetrate strips, polyacrylamide gel, or a capillary tube. Many typse of samples use open capillary tubes, other supports are usually used for biological samples (i.e. protein mixtures or DNA fragments. Post-Completion of separation is sustained in order to visualize the separated components. Using resolution will largely improvewhen using isoelectric focusing. Using resolution, the supported gel maintains a pH gradient. Asprotein migratesdown te gell, they reach a pH that is equal to its isoelectric point. Arriving at this pH gradient, the prtein is neutral and no longer migrates.

Schematic of zone electrophoresis apparatus
eleczone.gif

Specific electrophoretic techniques
  • disc electrophoresis
  • capillary electrophoresis
  • gel electrophoresis (SDS-PAGE) (1)
    • Widely used as a technique for separating electrically charged molecules, this process is a central technique in molecular biology and genetics laboratories, because it allows researchers separate and puify the nucleic acids DNA, RNA and proteins. This will allow them to be studied individually. Gel electrophoresis is often followed by staining or blotting procedures which are used to identify the separated molecules.(2)

Gel Elecrtiphorisis Equipment:
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References:
(1) http://elchem.kaist.ac.kr/vt/chem-ed/sep/electrop/electrop.htm
(2) http://www.answers.com/topic/gel-electrophoresis?cat=technology